Let-7b miRNA expression in Patients with Iron Deficiency Anemia after Bariatric surgery.

Document Type : Original Clinical

Authors

1 Department of Internal Medicine, Faculty of Medicine, Zagazig University, Egypt.

2 Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt

3 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez University, P.O. Box: 43221, Suez, Egypt.

Abstract

Aims: This study aimed to evaluate the expression of miR-let-7b in patients with anemia after bariatric surgery. Also, to investigate the correlation between miR-Let-7b expression and iron profile and copper serum levels.
Patients and Methods: This study included 50 participants from both sexes divided into 25 anemic subjects and 25 healthy individuals. All participants underwent entire history taking, thorough clinical examination, and laboratory investigations, including serum copper and iron measurements. Serum miR-let-7b expression was estimated by real-time polymerase chain reaction.
Results: There was a statistically significant increase in miR-let-7b expression in anemic patients after bariatric surgery compared to healthy individuals. Also, there were highly statistically significant negative correlations between miR-let-7b expression and each of serum iron (r=-0.883, P<0.001) and serum copper (r=-0.797, P<0.001). Receiver operating characteristic (ROC) curve results for miR-let-7b expression demonstrated that at a cut-off> 1.22, the area under the curve (AUC) equals 1.0 (P <0.001), the sensitivity was 100%, the specificity was 98%, positive predictive value (PPV) was 96.6%, negative predictive value (NPV) was 100%, and accuracy was 98%.
Conclusions: Mir-let-7b upregulation is associated with iron and copper deficiency in anemic patients after bariatric surgery. 

Keywords

Footnotes. Peer-Reviewers: Amany Mohamed Abdallah (Assistant professor of community medicine), Ahmed Fathy (Assistant professor of internal medicine), Neveen Fouad (assistant professor of internal medicine). E- Editor: Salem Youssef Mohamed, Osama Ahmed Khalil, Mohamed Hassan Ali Emara. Copyright ©. This open-access article is distributed under the Creative Commons Attribution License (CC BY). The use, distribution, or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited. The original publication in this journal is cited by accepted academic practice. No use, distribution, or reproduction is permitted, complying with these terms. Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent their affiliated organizations or those of the publisher, the editors, and the reviewers. Any product evaluated in this article or its manufacturer's claim is not guaranteed or endorsed by the publisher. Ethics Approval and Consent to Participate: The Internal Medicine Department and the Institutional Review Board Committee, Zagazig University (ZU-IRB#4740/25-7-2018) approved the experimental protocol. Consent for publication: All patients included in this research gave written informed permission to publish the data contained within this study. Availability of data and materials: The datasets used or analyzed during the current study are available from the corresponding author upon reasonable request. Competing interests: The authors declare that they have no competing interests. Funding: This study had no funding from any resource. Authors' contributions AF and SH were responsible for conception and revision, and AAYA and SH were responsible for the interpretation and analysis of data. SH wrote the manuscript that was revised and approved by all co-authors.

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