The influence of lncRNA expression dysregulation on predicting celiac diseases among patients with Hashimoto's thyroiditis

Document Type : Original Clinical

Authors

1 Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt.

2 Tropical Medicine department, Faculty of Medicine, Zagazig University, Egypt.

3 Medical Microbiology and Immunology Department, faculty of medicine, Zagazig University, Egypt.

4 Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt.

5 Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt.

Abstract

Aim: the study aimed to investigate lncRNA IFNG-AS1 in Hashimoto’s thyroiditis (HT) and to evaluate its predictive and diagnostic values of Celiac disease (CD) among patients with HT. Patients and Methods One hundred voluntary subjects: 50 healthy controls and 50 patients with HT;39 without CD and 11 with CD. Routine labs, including anti-TG, anti-TPO, and anti-TTG-IgA, investigated all subjects. lncRNA IFNG-AS1 expression was analyzed using Quantitative real-time RT-PCR.
Results
Patients with CD had significantly overexpressed lncRNA IFNG-AS1(4.17±0.618) compared to patients without CD (1.92±0.52) and control group (0.98±0.21), p ˂0.001*. It correlated considerably with intestinal and extra-intestinal manifestations and laboratory tests, including anti-TG, anti-TPO, and TTG IgA. Multivariate regression confirmed that the only variables independently associated with lncRNA IFNG-AS1 in the prediction of CD among studied parameters were anti-TG, anti-TPO, and TTG IgA. We found high sensitivity and specificity of 96% and 94% among HT, respectively. 
Concerning CD, the predictive power of IFNG-AS1 had sensitivity and specificity of 63.3% and 53%, respectively.
Conclusion: lncRNA IFNG-AS1 level was overexpressed in patients with HT, particularly those with CD, and correlated to clinical and laboratory parameters. Accordingly, it may be a promising predictive biomarker for HT and CD.

Keywords

Footnotes. Peer-Reviewers: Amany Mohamed Abdallah (Assistant professor of community medicine), Marwa Shabana (Assistant professor of clinical pathology), Mohamed Emara (professor of gastroenterology, hepatology, and infectious diseases). E- Editor: Salem Youssef Mohamed, Osama Ahmed Khalil, Mohamed Hassan Ali Emara. Copyright ©. This open-access article is distributed under the Creative Commons Attribution License (CC BY). The use, distribution, or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited. The original publication in this journal is cited by accepted academic practice. No use, distribution, or reproduction is permitted, complying with these terms. Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent their affiliated organizations or those of the publisher, the editors, and the reviewers. Any product evaluated in this article or its manufacturer's claim is not guaranteed or endorsed by the publisher. Ethics Approval and Consent to Participate: The Internal Medicine Department and the Institutional Review Board Committee, Zagazig University approved the experimental protocol. Consent for publication: All patients included in this research gave written informed permission to publish the data contained within this study. Availability of data and materials: The datasets used or analyzed during the current study are available from the corresponding author upon reasonable request. Competing interests: The authors declare that they have no competing interests. Funding: This study had no funding from any resource. Authors’ contributions N M. R, M O W were responsible for conception and revision, and A M S, M H. S were accountable for the interpretation and analysis of data. M H.S. H, A S, A T H wrote the manuscript that was revised and approved by all co-authors.

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